Download Advances in Bioceramics and Porous Ceramics II by Roger Narayan, Paolo Colombo, Dileep Singh, Jonathan Salem PDF

By Roger Narayan, Paolo Colombo, Dileep Singh, Jonathan Salem

Enhance your figuring out within the most precious features of advances in bioceramics and porous ceramics. This selection of logically prepared and thoroughly chosen articles comprise the complaints of the “Porous Ceramics: Novel advancements and purposes” and “Next iteration Bioceramics” symposia, that have been hung on January 27-February 1, 2008.

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The cell suspensions were seeded in triplicate on the eCA and eCA-nHA scaffolds, as well as empty control wells, in order to study cell attachment and proliferation. The seeding density of cells was approximately 68,000 cellsiwell for the PicoGreen assay and 50,000 cellsiwell for the MTS assay. The cells were incubated with the scaffolds for 1 and 3 days before analysis. Pic0 Green Assay The proliferative ability of cells was studied using a fluorescent nucleic acid dye that quantifies double stranded DNA (dsDNA) (Quant-iTTMPicoGreen' Kit, Invitrogen).

Then, 100 pL of the PicoGreen@reagent was added to 100 pL of the cell lysate in a 96-well plate before measuring fluorescence in the fluorescence microplate reader [ 161. MTS Assay The metabolic activity of cells was monitored using a tetrazolium compound (MTS) that is chemically reduced by metabolically active cells into formazan (CellTiter 96@ Aqueous One Solution Cell Proliferation Assay, Promega, Madison, WI). 'The measurement of formazan absorbance was performed in 96 well plates after 1 and 3 days incubation [17].

In addition, autologous bone grafts have shown considerable resorption and limited viability because of the lack of vascularization. 2. Allogenic bone grafts obtained from tissue banks also have limitations because of the possible transmission of non-conventional agents or viruses and the risk of immunological incompatibility [2,3]. Allogenic bone grafts have both osteoinductive properties (they release morphogenic bone proteins that act on bone cells) and osteoconductive properties, but lack osteogenic properties because of the absence of viable cells [4].

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